Clostridium butyricum|357821 |BNCC

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Clostridium tyrobutyricum

Culture medium	BNCC360846
Description	Modified GAM Broth
Composition	Peptone:5.0g, Tryptone:5.0g, Soy Peptone:3.0g, Yeast Extract Powder:2.5g, Beef Powder:2.2g, Digested Serum Powder:10.0g, Beef Liver Powder:1.2g, Glucose:0.5g, KH₂PO₄:2.5g, NaCl:3.0g, Soluble Starch:5.0g, L-Cysteine:0.3g, L-Arginine:1.0g, L-Tryptophan:0.2g, Sodium Thioglycolate(C₂H₃NaO₂S):0.3g, Sterile 0.1% Vitamin K1 Solution:5mL, Sterile Hemin:10mg, pH:7.3±0.1(25℃)
Growth conditions	37 ° C; 4-5 days; anaerobic;
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: Pale yellow Protrusion: Flat Surface: Rough Texture: Moist and easily lifted
Sharing mode	Public welfare sharing