BeNa Culture Collection
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| Culture medium | BNCC364352 |
| Description | ATCC Medium 329: Mineral Salts Agar |
| Composition | NaNO3:2.0g, MgSO4:0.5g, KCl:0.5g, Fe2(SO4)3.H2O:0.01g, KH2PO4:0.14g, K2HPO4:1.2g, Agar:15.0g, Yeast Extract:0.02g, pH:7.2(25℃) |
| Growth conditions | 28 ℃; 5-7 days; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Growing on ATCC329 mineral salt agar medium, the colony is low and flat, light green, and produces black brown ascocarps |
| Sharing mode | Public welfare sharing |
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