Vibrio harzianum|336937 |BNCC

Essential Information References Certificate Related Products

Vibrio harveyi

Subculture procedure	① Dissolve the Frozen vial in a water bath (30 ℃) and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	30 ℃; 18-24h; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, semi transparent, protruding in the middle, smooth surface, moist texture, easy to pick up, G - (red), Vibrio species
Sharing mode	Public welfare sharing

1.Description

1. Name:Vibrio harveyi

2. BNCC No.:336937

3. Biosafety level : 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, TSA + 5% defibrinated sheep blood: tryptone (casein trypsin digest) 15.0g, soybean peptone (soybean powder papain digest) 5.0g, sodium chloride 5.0g, agar 15.0g,pH7.3±0.2. 121 ℃,15min. After sterilization, 5% defiber sheep blood was added under aseptic conditions. Or sea water agar 2216.

2.Atmosphere:aerobic

3. Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

Download certificate