Bacillus subtilis 5230 [NRS 6]|338176 |BNCC

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Bacillus subtilis (Ehrenberg) Cohn

Culture medium	BNCC352232
Description	Tryptic Soy Broth
Composition	Tryptone:17.0g, Soy Peptone:3.0g, NaCl:5.0g, K₂HPO₄:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃)
Growth conditions	30 ℃; 18-24 hours; aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony has a diameter of 2-4mm, irregular shape, irregular edges, opacity, gray white front, flat shape, rough surface, hard texture, dry texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing