BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360993 |
| Description | LB Medium with 50μg/mL Chloramphenicol |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, Chloramphenicol:50μg/mL, pH:7.0(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② Open the safety cabinet and follow the arrow indication on the cap of the penicillin bottle to open the strain and reconstitution solution; ③ Add 1mL of the complex solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Take 200 μ L of bacterial suspension separately and inject it into two plates, apply evenly; ⑤ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | -20 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, moist texture, easy to pick |
| Sharing mode | Public welfare sharing |
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