Escherichia coli expression bacterium Rosetta gami (DE3) pLysS|357925 |BNCC

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Rosetta-gami(DE3)pLysS

Culture medium	BNCC391487
Description	LB Agar Plate with 50μg/mL Ampicillin, 50μg/mL Kanamycin, 50μg/mL Streptomycin, and 10μg/mL Tetracycline (Ready-to-use)
Composition	Pancreatic Digest of Casein:5.0g, Peptone:5.0g, Yeast Extract:10.0g, Beef Extract:5.0g, Glucose:5.0g, K₂HPO₄:2.0g, Tween 80:1.0ml, CaCl₂.2H₂O:0.01g, MgSO₄.7H₂O:0.02g, K₂HPO₄:0.04g, KH₂PO₄:0.04g, NaHCO₃:0.4g, NaCl:0.08g, Resazurin:1.0mg, Hemin:5mg, Vitamin K1:1mg, C₃H₇NO₂S.HCl:0.5g, Rumen Fluid(30ml/L), pH:7.2±0.2(25℃)
Growth conditions	37 ℃; 24 hours; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, light yellow on the front, smooth and bright on the surface, moist texture, no pigment, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing