DH10Bac Escherichia coli|353824 |BNCC

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Escherichia coli DH10Bac

Culture medium	BNCC352243
Description	LB nutrient agar
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0±0.2(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	1. Prepare one sterile liquid culture tube (containing 5–10 mL of medium) and two 90 mm agar plates. 2. After disinfecting the outer wall of the vial, open the aluminum cap and rubber stopper under aseptic conditions. Aspirate approximately 0.5 mL of liquid medium and inject it into the vial, gently pipetting to dissolve the bacterial pellet. 3. Once the pellet is fully dissolved, aspirate the entire suspension and transfer it back into the liquid culture tube, mixing thoroughly. 4. From the liquid culture tube, aspirate the mixed suspension and spread it onto the agar plates (200 μL per Agar plate). 5. Place both the tube and the plates under the specified incubation conditions. Do not seal or wrap the plates. After 18–24 hours, observe for visible turbidity in the liquid culture and colony/lawn growth on the plates. 6. If no growth is observed, continue incubation up to 72 hours. Once growth is confirmed, the culture can be used according to experimental requirements.
Storage conditions	-20 ℃
Safety level	1
morphology	Growth on the streak Agar plate.
Sharing mode	Public welfare sharing