Pichia pastoris KM71|361963 |BNCC

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Pichia sp KM71

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	30 ℃; 24-48 hours; aerobic
Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Colonies were 2-4 mm in diameter, circular with an entire margin, and opaque. They were creamy-white in color, convex in elevation, and had a smooth, glistening, moist, and easily liftable surface. Microscopic examination revealed Gram-positive (blue-violet) cocci. The culture was pure.
Sharing mode	Public welfare sharing