Chicken-derived nucleic acid detection kit certificate

1. Product information

Sample name: Chicken-derived nucleic acid detection kit (fluorescence PCR method)

Sample number: 361134

Sample batch: 220329

2. Product features

3. Instruction

Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, and then place on ice for later use. 25μL of experimental system: 24μL of qPCR mixed reaction solution, 1μL of template; recommended reaction conditions: the first step is 95℃ for 1min, the second step is 95℃ for 10s, the third step is 50℃ for 30s, and the second to third steps are cycled 40 times; fluorescence channel select Cy5.

4.Result judgment

Negative: no exponential growth curve, or low growth curve and Ct value>35; Positive: obvious exponential growth curve, and Ct value ≤35;

5. Notes

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly, and then place on ice for use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833