Certificate of Enterobacter aerogenes nucleic acid detection kit

1. Product information

Sample name: Enterobacter aerogenes nucleic acid detection kit (fluorescence PCR method)

Sample number: 361454

Sample batch: 2200505

2. Product features

3. Instructions

Experiment preparation: Carry out enrichment culture of samples according to GB 4789.41-2016 before the experiment; dissolve various components in the kit on ice before use, mix lightly and centrifuge briefly and place on ice for later use; Reagent preparation: experimental reaction System (25 μL), 15.3 μL of qPCR mixed reaction solution, 1 μL of template, 8.7 μL of sterile ddH2O. Reaction conditions: 95°C for 1min 1cycle; 95°C for 10s, 57.4°C for 30s for 40cycles; Fluorescence channel: VIC.

4. Notes

1. The kit should be stored at low temperature in the dark, and the various components in the kit should be thawed naturally before use, mixed well and centrifuged briefly to avoid the generation of air bubbles;

2. Sample processing, reagent preparation, and sample addition should be performed in different areas to avoid cross-contamination;

3. Judgment of results: If the Ct value of the test sample is ≥38 and there is no typical amplification curve, it is judged as negative; if the Ct value of the test sample is less than or equal to 35, and two typical amplification curves appear, it is judged as positive; If the Ct value of the test sample is >35 and <38, it is recommended that the sample be redone.

5. Preservation and transportation

long-term storage:-20 ℃, valid for 1 year; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833