Shigella nucleic acid test kit certificate

1. Product information

Sample name: Shigella nucleic acid detection kit (fluorescent PCR method)

Sample number:  361199

Sample batch: 220402

2. Product features

3. Instructions

Sample processing: sample processing according to《GB/T 4789.5-2003 Shigella in Food》;

Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;

Reagent preparation: experimental reaction system (25 μL), 15 μL qPCR mixed reaction solution, 1 μL template, 9 μL sterile ddH2O (10 μL when using diluted bacterial solution as template);

Reaction conditions: 95°C 1min 1cycle; 95°C 10s, 52°C 30s 40cycles (when the diluted bacterial solution is used as the template for the first step reaction for 3min);

Fluorescence channel: FAM;

4. Precautions

Negative: The sample has no exponential growth curve, or has a lower amplification curve, and the Ct value is >35;

Positive: The sample has a typical exponential growth curve, and the Ct value is ≤35;

5. Preservation and transportation

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Research Center of Industrial Microbiology

Website: www.bncc .org .cn Tel: 400-6699-833