Listeria monocytogenes nucleic acid test kit certificate

1. Product information

Sample name: Listeria monocytogenes nucleic acid detection kit (fluorescent PCR method)

Sample number:  361444

Sample batch: 220505

2. Product features

3. Instructions

Sample processing: sample processing in accordance with 《GB 4789.30-2016 National Food Safety Standard - Detection of Listeria monocytogenes》;

Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;

Reagent preparation: experimental reaction system (25 μL), 15 μL qPCR mixed reaction solution, 1 μL template, 9 μL sterile ddH2O

Reaction conditions: 95℃ 1min 1cycle; 95℃ 10s, 57℃ 30s 40cycles; Fluorescence channel: FAM;

4. Notes

Negative: The sample has no exponential growth curve, or has a lower amplification curve, and the Ct value is >38;

Positive: the sample has a typical exponential growth curve, and the Ct value is ≤38;

5. Preservation and transportation

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Research Center of Industrial Microbiology

website: www.bncc .org .cn tel: 400-6699-833