Certificate of Pseudomonas aeruginosa Nucleic Acid Detection Kit

1. Product information

Sample name: Pseudomonas aeruginosa nucleic acid detection kit (fluorescence PCR method)

Sample number: 361453

Sample batch: 2200505

2. Product features

3. Instructions

Sample processing: Refer to "SN/T 5228.9-2019 Rapid Screening Method of Pathogenic Microorganisms in Exported Foods for Pseudomonas aeruginosa" for enrichment culture; Experiment preparation: Dissolve various components in the kit on ice before use, slightly Mix well, centrifuge briefly, and place on ice for later use; Reagent preparation: experimental reaction system (25 μL), 18.5 μL of qPCR mixed reaction solution, 1 μL of template, 5.5 μL of sterile ddH2O; Reaction conditions: 95℃ for 1min 1cycle; ℃ 30s 40cycle; Fluorescence channel: FAM.

4. Notes

1. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

2. To ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Judgment of results: if the Ct value of the test sample is greater than or equal to 40 and there is no typical amplification curve, it is judged as negative; if the Ct value of the test sample is less than or equal to 40 and there is a typical amplification curve, it is judged as positive; if the test sample is tested Ct values >35 and <40 suggest that the sample be redone.

5. Preservation and transportation

long-term storage:-20 ℃, valid for 1 year; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833