Certificate of Human Adenovirus Type 41 Nucleic Acid Detection Kit

1. Product information

Sample name: human adenovirus type 41 nucleic acid detection kit (fluorescent PCR method)

Sample number: 361556

Sample batch: 220513

2. Product features

3. Instruction

Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;

Experimental reaction system (25 μL): 17 μL qPCR mixed reaction solution, 2 μL template, and sterile ddH2O to make up to 25 μL;

Experimental reaction conditions: the first step is 95℃ for 1min, the second step is 95℃ for 10s, the third step is 60℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM.

4. Result judgment

Negative: no exponential growth curve, or a lower growth curve and a Ct value >35;

Positive: There is an obvious exponential growth curve, and the Ct value is ≤35.

5. Notes

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833