Reference material of Aspergillus niger Spores Count

Product number: 359090

Production batch:20210621

Date of setting value: June 15, 2021

In recent years, the application of probiotic products has become more and more extensive. Aspergillus niger, as a probiotic allowed by the country, has also been widely used in livestock and poultry breeding and organic fertilizer fermentation and other related industries, and its effect has been generally recognized. This reference material provides a physical standard for aspergillus niger spore counting. Aspergillus niger grows to produce spores, collects spores, and freezes after adding suitable stabilizers. It is applicable to "Technical Specification for Disinfection" (Ministry of Health, 2002 Edition), WS/T 683-2020 《Microbial Requirements for Disinfection Test》 and GB 4789.15-2016 《Mold and Yeast Count of National Food Safety Standard Food Microbiological Test》.

1.Ssample preparation

Select and culture Aspergillus niger (Aspergillus niger, strain number CMCC98003) to  produces spores, and dispense the spore suspension into penicillin bottles by pasteur pipette, then make it freeze-dried and sealed.

2. Traceability and value setting method

The reference material is determined by multiple organizations using the spreading method. After 10 times series dilution of the standard substance, the number of colonies on the medium plate is counted, and the total number of colonies is calculated by formula, and the unit of measure is CFU/mL(CFU, colony forming unit Colony-Forming Units). By using quality volume etc measurement instruments that is verified and calibrated, and the quantitative measurement methods which meet the requirements of metrology characteristics, it is ensured that the measurement value of this reference material can be traced back to the basic unit of entity number “1” and the national legal unit of measurement “L”.

3. Characteristic value and uncertainty

The uncertainty of the setting value is obtained by the combination of uncertainty components introduced by the setting method, balance, pipette, dilution factor, uniformity and stability.

4. Uniformity and stability evaluation

According to the JJF1343 national metrology technical specifications (equivalent ISO guide 35), use Aspergillus niger spore counting and spreading method, and evaluate the uniformity of the sample according to the principle of random sampling. The results of variance analysis show that the uniformity of the sample is good; Using the same measurement method, the stability of the sample is evaluated according to the principle of first density and then sparse. The trend analysis results show that it can be stored stably for a long time at -20 ℃, for 7 days at 4 ℃ and can be shipped in ice bags. The reference material is valid for 12 months from the setting date. We will continue to track and monitor the stability of the standard substance. If changes in the value are found during the validity period, the user will be notified in time.

5. Packaging, storage and usage

1. packaging and preservation: the reference material is packed in 3ml penile bottles and sealed after freeze-drying. It is shipped on dry ice. Upon receipt, check if the dry ice is exhausted or not. Then put it in a refrigerator at -20 ℃.

2. use: the reference material should be used up at one time after opening the bottle. Before use, take it out of -20 ℃ and balance it at room temperature for 10 minutes. Use a pipette to accurately measure 1.0mL(20 ℃) of sterile distilled water to re-dissolve the freeze-dried reference material.

3. the reference material contains microorganisms. masks and latex gloves should be worn before handling. Pay attention to protect yourself during the handling, avoid inhalation or direct skin contact. After use, the remaining samples, packaging bottles, sampling tips and other consumables should be autoclaved according to biological pollution waste.

6. References

[1] GB 4789.15-2016 National Food Safety Standard for Microbiological Examination of Mold and Yeast Count in food[S].

Statement

1. the reference material is only for laboratory research and analysis and testing. Complaints arising from improper use or storage by users shall not be liable.

2. Please check the variety, quantity, packaging and certificate immediately upon receipt. The relevant compensation is limited to the reference material itself and does not involve any other losses.

3. the reference material is shipped with the certificate stamped”"special seal for certified reference materials of Beijing Beina Chuanglian Biotechnology Research Institute". We will not accept any complaints if the certificate can not be provided. Please keep this certificate properly.

4. For more application-related information, please contact the technical department.

Beijing Beina Chuanglian Biotechnology Research Institute

Technicians: 400-6699-833