Positive quality control sample of fecal coliforms in water quality (paper rapid method)|360408 |BNCC

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Water quality fecal coliform positive quality control samples (Paper Rapid Method)

Subculture procedure	The sample needs to be dissolved in a total of 10mL sterile water or sterile physiological saline. Before use, place the quality control sample at room temperature for 30 minutes, then open it in a biosafety cabinet and immediately add 10 mL of sterile water. After dissolution, shake the penicillin bottle and shake it evenly to obtain a positive quality control sample, totaling 10 mL with a concentration of 300-3000 CFU/mL. The sample needs to be dissolved in a total of 10 mL of sterile water or sterile physiological saline. Before use, place the quality control sample at room temperature for 30 minutes, then open it in a biosafety cabinet and immediately add 10 mL of sterile water. Dissolve it and shake the penicillin bottle evenly to obtain a positive quality control sample, totaling 10 mL with a concentration of 300-3000 CFU/mL. After receiving the goods, please be sure to keep them properly. If there is any damage or other situation, please contact our center within 24 hours after receiving them.
Growth conditions	Cultivation temperature 37 ℃; Cultivation time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, stored in the dark
Safety level	1
Sharing mode	Public welfare sharing

water quality fecal coliform positive quality control sample certificate

1. Product information

Sample name: water quality fecal coliform positive quality control sample (paper rapid method)

Sample number: BNCC 360408

sample batch: 211203

2. Product features

description	appearance is white powder (freeze-dried powder), containing protective agent, 7mL penile bottle packaging
traceability	ATCC 25922 Escherichia coli
concentration value	300~3000 CFU/mL
reference value	750 CFU/mL
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	"HJ 755-2015 Rapid Paper Method for Determination of Total Coliforms and Fecal Coliforms in Water Quality"
* Note: If you have any questions about quality control samples, please contact BNCC for help

3. Instructions

Dissolve the material by 6mL of sterile water or sterile saline. Before use, the quality control sample is placed at room temperature for 30min, it was then opened in a biological safety cabinet,immediately add 6 mL of sterile water, shake the vial after dissolving，evenly shaken is a positive quality control sample，the concentration is 300~3000 CFU/mL.

4. Precautions

1. Upon receipt, please keep it properly. if it is damaged, please contact us within 24 hours.

2. Please handle in a biosafety cabinet under sterile conditions. The material shall be used up once opened.

3. the product contains risk-3 pathogenic organisms，please do not directly touch the samples with your hands, please discard the residual samples after high temperature and high pressure sterilization.

5. Preservation and transportation

long-term storage: -20 ℃, valid for 1 year; ship on ice bag; the shipping time should not exceed 7 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc .org .cn tel: 400-6699-833

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