Fecal coliform negative control quality control sample (multi tube fermentation method)|386994 |BNCC

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Fecal coliform negative control quality control sample(Multi tube fermentation method)

Growth conditions	Cultivation temperature 37 ℃; Cultivation time 24-48 hours; Aerobic gas environment
Subculture procedure	The sample needs to be dissolved in a total of 300mL sterile water or sterile physiological saline.Prior to use, allow the quality control sample to equilibrate to room temperature for 30 minutes. Subsequently, open it within a biosafety cabinet, immediately add 3–4 mL of sterile water for reconstitution, and then aspirate and transfer the solution into a sterile bottle.Rinse the inner walls of the vial with the remaining sterile water, and transfer all washes to the sterile bottle mentioned above. The resulting solution constitutes the negative quality control sample, with a total volume of 300 mL and a concentration of 300–3000 MPN/L.
Storage conditions	-20 ℃, stored in the dark
Sharing mode	Public welfare sharing