Quantitative strain of Bacillus subtilis|353782 |BNCC

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Quantitative strains of bacillus subtilis

Growth conditions	37 ℃, aerobic
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, flat in shape, bright on the surface, dry in texture, difficult to pick/cut, G+(blue purple), Bacillus
Sharing mode	Public welfare sharing

Bacillus subtilis

No. :353782 / CMCC63501

Product format: quantitative bacteria tablets

Number of live bacteria : 100 ~ 500CFU/bottle

Product batch : 191212

Valid until : 2020.6.14

Date of inspection : 2019.12.15

Sampling ratio : ≥2%

Report number: RS19353782-1

Biosafety level : 1,handle in ultra-clean table or safety cabinet

item	description	test result
stain microscopy	Gram-positive, observe cell morphology under microscope	G + cocci
colony morphology	observation of single colony morphology	Gray, dry, flat, irregular edges
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	non-selective medium plate, live bacteria count	260CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list :1 bottle of 100 ~ 500CFU quantitative strain, 1 bottle of 1mL dissolved solution and 1 copy of strain certificate;

Validity period : unopened, below -20 ℃, 6 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 100-500cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Attentions:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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