Quantitative strain of Bacillus subtilis|361421 |BNCC

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Quantitative strains of bacillus subtilis subsp.

Growth conditions	30 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, flat in shape, rough on the surface, dry in texture, G+(blue purple), Bacillus
Sharing mode	Public welfare sharing

Bacillus subtilis subsp.

Strain number: BNCC 361421 /ATCC6633

Product format: quantitative bacteria tablets

Number of viable bacteria:(4.7±0.3)×10⁷CFU/bottle

Product batch: 220428

Valid until: 2023.04.27

Date of inspection: 2022.04.27

Sampling ratio:≥2%

Report number: RS22293727-1

Biosafety level: 1, handle in ultra-clear table or safety cabinet

items	description	results
stain microscopy	Gram-negative, positive, cell morphology observed under microscope	G + cocci
colony morphology	Observe the morphology of single colonies	golden yellow, raised, bright, moist
purity	Non-selective culture medium, colony observation and microscopy	No contaminated bacterial
Reference value of live bacteria	Non-selective media plates, viable count	(4.7±0.3)×10⁷CFU
Phenotypic and Genotypic Analysis	Phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system, the product is qualified

Product list: quantitative strains 1 bottle, 1mL lysate 1 bottle, 1 strain certificate;

Validity period: unopened, below -20 ℃, 12 months.

Usage:

1. Open the strain and lysate according to the arrow indication of the vial cap;

2. Pour the bacterial flakes into the dissolving solution, cover with a rubber stopper, and shake to dissolve;

3. After fully dissolved, it is (4.7±0.3)×10⁷CFU/mL, and it can be drawn in proportion according to the test requirements;

4. Please use up immediately after opening and do not store;

Notes:

① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.

②Used germ-carrying consumables should be discarded after autoclaving.

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