Quantitative strains of Bacillus subtilis|353544 |BNCC

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Quantitative strains of bacillus subtilis subsp.

Growth conditions	30°C; 18-24h; Aerobic
Storage conditions	-20°C
Safety level	1
morphology	Colony diameter: 2-4 mm, circular, wavy-edged, opaque, grayish-white upper surface, centrally raised, wrinkled surface, dry texture, easily lifted, G+ (blue-violet), Bacillus
Sharing mode	Public welfare sharing

Bacillus subtilis

No. : 353544 / ATCC 6633

Product format: quantitative bacteria tablets

Number of live bacteria : 100 ~ 500CFU/bottle batch: 190916

Valid until : 2020.9.15

Date of inspection : 2019.9.16

Sampling ratio : ≥2%

Report number : RS19353544-2

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Item	description	test results
staining microscopy	gram-negative and positive, cell morphology observed under microscope	G + bacillus, in the shape of single rod, double rod and connecting rod
colony morphology	observation of single colony morphology	light pink, rough, low flat, irregular edges
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	non-selective medium plate, live bacteria count	360CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list :1 bottle of 100 ~ 500CFU quantitative strain, 1 bottle of 1mL dissolved solution and 1 copy of strain certificate;

Validity period : unopened, below -20 ℃, 12 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 100-500cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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