Quantitative strain of Staphylococcus aureus|359608 |BNCC

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Quantitative strains of staphylococcus aureus

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	Round, with neat edges, opaque, golden yellow on the front, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Staphylococcus aureus

Strain number: BNCC 359608 / CMCC 26003

Product format: quantitative bacteria tablets

Product batch:2021.08.26

Date of inspection:2021.08.27

Valid until:2023.08.25

Number of viable bacteria:50-100CFU/bottle

Sampling ratio:≥2%

Report number:RS22359608-1

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Items	test results	description
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + bacilli
colony morphology	observation of single colony morphology	golden yellow, raised, moist, with neat edges
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	Non-selective medium plate, viable count	51CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list:1 bottle of 50-100CFU quantitative strain, 1 bottle of 1mL lysate, 1 strain certificate;

Validity period: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After fully dissolved, it is 50- 100CFU/mL, which is absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.

②Used germ-carrying consumables should be discarded after autoclaving.

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