Quantitative Strain of Escherichia coli|354945 |BNCC

Essential Information Certificate Related Products

Quantitative strains of escherichia coli (Migula) Castellani and Chalmers

Subculture procedure	① Cultivate bacteria into suspension according to culture conditions; ② Measure suspension absorbance and calculate initial concentration using standard formula; ③ Multiply initial concentration by survival rate to obtain actual lyophilized concentration;④ Dilute the bacterial suspension to target concentration based on the actual post-lyophilization concentration, then aliquot and freeze-dry;
Growth conditions	37°C; 18-24h; Aerobic;
Storage conditions	-20°C
Safety level	1
morphology	Colony diameter 1-2mm, circular, with neat edges, opaque, grayish-white upper surface, raised center, smooth surface, glossy appearance, moist texture, easily lifted, G− (red), bacillus
Sharing mode	Public welfare sharing

Escherichia coli

Strain number:BNCC 354945 / ATCC25922

Name: Escherichia coli

Product format: quantitative bacteria tablets Number of viable bacteria: 300 ~ 500CFU/bottle

Product batch: 21122001 Valid until: 2022.12.19

Inspection date: 2021. 12.21 Sampling ratio: ≥2%

Report number: RS21354945- 1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

Item	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli
colony morphology	observation of single colony morphology	pale yellow, raised, bright
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	380CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

Product list: 300 ~ 500CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Validity period: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After fully dissolving, it is 300 ~ 500CFU/bottle, which can be sucked in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

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