Streptococcus salivarius Quantitative Strain|354947 |BNCC

Essential Information Certificate Related Products

Quantitative strains of streptococcus salivarius

Subculture procedure	① First cultivate bacteria into a bacterial suspension under specified conditions; ② Measure the absorbance of the bacterial suspension and calculate its initial concentration using the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension to the target concentration based on the actual post-lyophilization concentration, then aliquot and lyophilize;
Growth conditions	37°C; 18-24h, aerobic;
Storage conditions	-20°C
Safety level	1
morphology	Colony diameter: 1-2mm, circular, with neat edges; opaque, grayish-white on both sides; centrally raised, smooth surface, glossy appearance, moist texture, easily lifted; G+ (blue-violet), cocci; Purity: Pure
Sharing mode	Public welfare sharing

Streptococcus salivarius

Strain number: BNCC 354947

Name: Streptococcus salivarius

Product format: quantitative bacteria tablets Number of viable bacteria: (2.5±1.0)×10^6CFU/bottle

Product batch: 21122001 Valid until: 2022.12.19

Inspection date: 2021.12.21 Sampling ratio: ≥2%

Report number:RS21354947-1 Biosafety level: 1,handle in ultra-clear table or safety cabinet

Item	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + cocci, spherical, chain-like arrangement
colony morphology	observation of single colony morphology	pale yellow, raised, moist, bright
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	2.0×10^6CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

Product list: One bottle of (2.5±1.0)×10^6CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;

Validity period: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After full dissolution, it is (2.5±1.0)×10^6CFU/bottle, according to the test requirements according to the proportion of suction can be;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

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