Quantitative strain of thermophilic fat loving Bacillus|354744 |BNCC

Essential Information Certificate Related Products

Quantitative strains of bacillus stearothermophilus

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	55 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, light color, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, does not produce pigments, G+(blue purple), Bacillus
Sharing mode	Public welfare sharing

Bacillus stearothermophilus

Strain number:BNCC 354744

Name: Bacillus stearothermophilus

Product format: quantitative bacteria tablets Number of viable bacteria: 1.0~2.5×10⁶CFU/bottle

Product batch:211210 Valid until: 2022.12.09

Inspection date: 2021.12.11 Sampling ratio: ≥2%

Report number:RS21336760-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

Item	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + bacilli, single, paired or irregular heap arrangement
colony morphology	observation of single colony morphology	Light yellow, rough folds, flat, irregular edges
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	2. 1×10⁶CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

Product list: 1 bottle of 1.0~2.5×10⁶CFU quantitative strain, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Shelf life: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. after fully dissolved, it is 1.0~2.5×10⁶CFU/bottle, which can be sucked in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes：

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

②Used bacteria-carrying consumables, please autoclave and discard them.

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