Quantitative strain of Candida albicans|353801 |BNCC

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Quantitative strains of candida albicans

Subculture procedure	① Cultivate the bacteria into bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	25-28 ℃, aerobic
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front and milky white on the back, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Candida albicans

No. : 353801 / CMCC(F)98001

Product format: quantitative bacteria tablets

Number of live bacteria : 1.0-1.5x10 3CFU/bottle

Product batch : 191218

Valid until : 2020.12.17

Date of inspection : 2019.12.20

Sampling ratio : ≥2%

Report number : RS19353801-1

Biosafety level :1 , handle in ultra-clean table or safety cabinet

item	description	test results
stain microscopy	gram-negative and positive, cell morphology observed under microscope	G + yeast, spherical
colony morphology	observation of single colony morphology	bright white, moist, raised, with neat edges
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	non-selective medium plate, live bacteria count	1120CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list :1.0-1. 5x103CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Validity period : unopened, below -20 ℃, 12 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 1.0-1.5x10 3cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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