Quantitative strain of Staphylococcus aureus|361282 |BNCC

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Quantitative strains of staphylococcus aureus

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	37 ℃, 18h, aerobic
Storage conditions	-20 ℃
Safety level	1
morphology	Round, with neat edges, opaque, golden yellow on the front, light color, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, non pigmented, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Staphylococcus aureus

Strain number: BNCC 361282 / CMCC 26003

Product format: quantitative bacteria tablets

Number of viable bacteria: (2.0±0.5)×10^6 CFU/bottle

Product batch: 220420

Valid until: 2024.04.19

Date of inspection: 2022.04.21

Sampling ratio:≥2%

Report number: RS22361282-1

Biosafety level: 2, handle in biosafety cabinet

items	description	results
stain microscopy	Gram-negative, positive, cell morphology observed under microscope	G + cocci
colony morphology	Observe the morphology of single colonies	golden yellow, raised, bright, moist
purity	Non-selective culture medium, colony observation and microscopy	No contaminated bacterial
Reference value of live bacteria	Non-selective media plates, viable count	2.05×10^6CFU/bottle
Phenotypic and Genotypic Analysis	Phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system, the product is qualified

Product list:(2.0±0.5)×10^6 CFU quantitative strains 1 bottle, 1mL lysate 1 bottle, 1 strain certificate;

Validity period: unopened, below -20 ℃, 24 months.

Usage:

1. Open the strain and lysate according to the arrow indication of the vial cap;

2. Pour the bacterial flakes into the dissolving solution, cover with a rubber stopper, and shake to dissolve;

3. After fully dissolved, it is (2.0±0.5)×10^6CFU/mL, and it can be drawn in proportion according to the test requirements;

4. Please use up immediately after opening and do not store;

Notes:

① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.

②Used germ-carrying consumables should be discarded after autoclaving.

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