Quantitative strain of Salmonella typhimurium in colitis mice|353547 |BNCC

Essential Information Certificate Related Products

Quantitative strains of Salmonella enterica subsp. enterica serovar Typhimurium

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	37 ° C, 18-24h; Aerobic
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus
Sharing mode	Public welfare sharing

Salmonella enterica

No.: 353547 / ATCC 14028

Product format: quantitative bacteria tablets

Number of viable bacteria : 0.5~1.0×10 3CFU/bottle

Product batch : 190916

Valid until : 2020.3.15

Date of inspection : 2019.9.16

Sample scale : ≥2%

Report number : RS19353547-1

Biosafety level : 2 , handle in safety cabinet

item	description	test result
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli
colony morphology	observation of single colony morphology	light yellow, raised, bright, round
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	Non-selective medium plate, viable count	870CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list :0.5~1.0 & times;1 bottle of 10 3CFU quantitative strain, 1 bottle of 1mL dissolved solution and 1 copy of strain certificate;

Validity period : unopened, below -20 ℃, 6 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 it will be 0.5~1.0×10 3cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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