Quantitative strain of Bacillus subtilis|353859 |BNCC

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Quantitative strains of bacillus subtilis subsp. spizizenii Nakamura et al.

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light in color, raised in the middle, smooth on the surface, bright on the surface, moist in texture, easy to pick up, does not produce pigments, G+(blue purple), Bacillus, purity: Pure
Sharing mode	Public welfare sharing

Bacillus subtilis

No.:353859 / ATCC 6633

Product format: quantitative bacteria tablets

Number of viable bacteria : 0.5~1.0×10 4CFU/bottle

Product batch: 191227

Valid until : 2020.12.26

Date of inspection: 2019.12.29

Sampling ratio : ≥2%

Report number : RS19353859-1

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

item	description	test result
stain microscopy	Gram-positive, observe cell morphology under microscope	G + cocci
colony morphology	observation of single colony morphology	Gray, dry, flat, irregular edges
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	non-selective medium plate, live bacteria count	9040CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list :One bottle of 0.5~1.0×10 4CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;

Validity period: unopened, below -20 ℃, 12 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 0.5~1.0x10 4 cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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