Quantitative strain of Escherichia coli|354966 |BNCC

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Quantitative strains of escherichia coli

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	Round, with neat edges, opaque, light yellow on the front, raised in the middle, bright and smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Escherichia coli

Strain number:BNCC 354966 / CMCC 44102

Name: Escherichia coli

Product format: quantitative bacteria tablets Number of viable bacteria: ( 1.8±0.2)×10^6CFU/bottle

Product batch: 220317 Valid until: 2024.03. 16

Inspection date: 2022.03.18 Sampling ratio: ≥2%

Report number: RS22354966-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

Item	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli
colony morphology	observation of single colony morphology	golden yellow, raised, moist, bright
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	1.8 × 10^6CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

Product list:One bottle of ( 1.8±0.2)×10^6CFU quantitative strain, one bottle of 1mL dissolution solution and one copy of strain certificate;

Shelf life: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. after full dissolution, it is (1.8±0.2)×10^6CFU/mL, can be absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

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