Quantitative strain of Escherichia coli|356000 |BNCC

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Quantitative strains of escherichia coli (Migula) Castellani and Chalmers

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, does not produce pigments, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Escherichia coli

Strain number:BNCC 356000/ATCC 8739

Name: Staphylococcus aureus subsp.

Product format: quantitative bacteria tablets Number of viable bacteria: (4.0±1.5)×10e⁷CFU/bottle

Product batch: 220608 Valid until: 2023.06.07

Inspection date: 2022.06.09 Sampling ratio: ≥2%

Report number: RS22356000-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

project	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli, in a single rod shape
colony morphology	observation of single colony morphology	milky white, raised, bright, or spread
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	4.5×10e ⁷CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

Product list: One bottle of (8.0±2.0)×10^6CFU quantitative strain, one bottle of 1mL dissolution solution and one copy of strain certificate;

Shelf life: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. after full dissolution, it is (4.0±1.5)×10e⁷CFU/bottle, which can be sucked in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

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