Quantitative strain of Escherichia coli|295929 |BNCC

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Quantitative strains of escherichia coli (Migula) Castellani and Chalmers

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, bright and smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus
Sharing mode	Public welfare sharing