BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ℃; 5-7 days; Aerobic |
| Subculture procedure | ① Culture the strain under specified conditions to obtain a bacterial suspension.② Measure the absorbance of the suspension and calculate its initial concentration using the standard formula.③ Multiply the initial concentration by the survival rate to determine the actual concentration after lyophilization.④ Dilute the suspension to the target concentration based on the post‑lyophilization concentration, followed by aliquotting and lyophilization. |
| Storage conditions | -20 ℃ |
| morphology | Initially, white mycelium develops; during later stages of cultivation, the mycelium begins to produce black spores. |
| Sharing mode | Public welfare sharing |
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