Quantitative strain of Staphylococcus aureus|356332 |BNCC

Essential Information Certificate Related Products

Quantitative strains of staphylococcus aureus

Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	Round, with neat edges, opaque, golden yellow on the front, light color, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, non pigmented, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Staphylococcus aureus

Strain number:BNCC 356332 / CMCC 26003

Name: Staphylococcus aureus

Product format: quantitative bacteria tablets Number of viable bacteria: (3.5±0.3)×10^4CFU/bottle

Product batch: 220420 Valid until: 2024.04 . 19

Inspection date: 2022.04 .21 Sampling ratio: ≥2%

Report number: RS22356332-1 Biosafety level: 2, handle in safety cabinet

project	description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + cocci
colony morphology	observation of single colony morphology	golden yellow, raised, moist, bright
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	3.5 × 10^4CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	qualified

Product list: One bottle of (3.5±0.3)×10^4CFU quantitative strain, one bottle of 1mL dissolution solution and one copy of strain certificate;

Shelf life: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After full dissolution, it is (3.5±0.3)×10^4CFU/bottle, according to the test requirements according to the proportion of suction can be;

4. Please use up immediately after opening, and do not store it;

Notes:

①Tthis product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

Download certificate