ε15|373642 |BNCC

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ε15

Culture medium	BNCC352252
Description	Brain Heart Infusion Agar
Composition	Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:13.5g, pH:7.4±0.2(25℃)
Growth conditions	37℃； 18-24h; aerobic;
Subculture procedure	① Host bacterial culture: Inoculate the host bacteria into the above culture medium and culture under the above conditions until they grow. ② Host bacteria preparation: Pick the host bacteria from the Agar plate, dissolve them in 40-50mL nutrient broth culture medium, shake at 37 ℃ and 160-180rpm until OD600 is 0.1-0.4; ③ Open the freeze-drying tube: Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ④ Dissolve Freeze dried pellet: Take 0.5mL of nutrient broth and transfer it into a freeze-drying tube. After sufficient dissolution, transfer it into a triangular bottle of host bacterial solution; ⑤ Phage culture: Place the triangular flask at 37 ℃ and incubate overnight on a shaker at 160-180rpm. After 16-18 hours, centrifuge the phage culture at 4000g for 10 minutes and filter the lysate through a 0.2 μ m sterile filter. The filtrate can be stored at 4 ℃
Storage conditions	-80 ℃
Sharing mode	Public welfare sharing