Escherichia coli bacteriophage T4|251275 |BNCC

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Escherichia coli bacteriophage T4-BNCC
  • Escherichia coli bacteriophage T4-BNCC
  • Escherichia coli bacteriophage T4-BNCC

Escherichia coli bacteriophage T4

  • Price： $ 223
  • number：BNCC251275
  • Packing：Bacterial solution
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Escherichia coli bacteriophage T4
Growth conditions 37 ℃; 18-24h; Aerobic
Subculture procedure Phage culture: ① Host bacterial preparation: Pick a single colony of host bacteria from a plate, dissolve it in 40-50mL nutrient broth culture medium, shake and culture at 37 ℃, 160-180 rpm until OD600 is around 0.4; ② Transfer: Remove the phage cryopreservation tube, rapidly melt it in a 37 ℃ water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 37 ℃ and shake at 160-180rpm overnight for cultivation; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and filter it through a 0.22 μ m sterile filter. Collect the filtrate, which can be stored at 4 ℃
Storage conditions -80 ℃
Sharing mode Public welfare sharing
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