Escherichia coli K-12 W.3350

Storage conditions: 2~8 ℃

No. 310053

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 10 days

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30 ℃, aerobic, nutrient agar medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 

(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; 

(3) Pick up 1-2 rings of plate colonies by inoculation loop, inoculate to fresh medium；

(4) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows: