Pseudomonas bacteriophage phi6|354296 |BNCC

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Pseudomonas phage phi6 (Cystovirus phi6)

Growth conditions	30 ℃; 24-48 hours; aerobic
Subculture procedure	phage culture: ① Host bacterial preparation: pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL tryptone soy broth culture medium, shake at 30 ℃, 160-180 rpm until OD600 is 0.4-0.6; ② Transfer: Remove the phage Frozen vial, rapidly melt it in a 30 ℃ water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 30 ℃ and shake at 160-180rpm for 48 hours; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and filter it with a 0.22 μ m sterile filter, and collect the filtrate. The filtrate can be stored at 4 ℃
Storage conditions	-80 ℃
Sharing mode	Public welfare sharing