Escherichia coli T1 phage|356259 |BNCC

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Escherichia phage T1

Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Host bacterial preparation: Pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth medium, shake and culture at 37 ℃, 160-180 rpm until OD600 is 0.1-0.4; ② Open the freeze-drying tube: Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Dissolve Freeze dried pellet: Take 0.5mL of nutrient broth and transfer it into a freeze-drying tube. After sufficient dissolution, transfer it into a triangular bottle of host bacterial solution; ④ Cultivation: Place the triangular flask at 37 ℃ and incubate overnight on a shaker at 160-180rpm; ⑤ Filtration: After 16-18 hours, centrifuge the phage culture at 4000g for 10 minutes. Filter the lysate using a 0.2 μ m sterile filter, which can be filtered twice to completely remove the host. The filtrate can be stored at 4 ℃
Storage conditions	2-8 ℃
Sharing mode	Public welfare sharing