Escherichia coli bacteriophage M13|364179 |BNCC

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Escherichia phage M13

Subculture procedure	phage culture: ① Host bacterial preparation: pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth culture medium, shake at 37 ℃, 160-180 rpm, and culture until OD600 is around 0.4; ② Transfer: Take an appropriate amount of bacterial solution and transfer it into a triangular bottle of host bacterial solution; ③ Cultivation: Place the triangular flask at 37 ℃ and incubate overnight on a shaker at 160-180rpm; ④ Filtration: After 16-18 hours, centrifuge the phage culture at 4000g for 10 minutes, filter the lysate using a 0.22 μ m sterile filter, and store the filtrate at 4 ℃
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	Liquid nitrogen
Safety level	2
Sharing mode	Public welfare sharing