BeNa Culture Collection
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| Growth conditions | 30 ℃; 18-24h; aerobic |
| Subculture procedure | phage culture: ① Host bacterial preparation: pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth culture medium, shake at 37 ℃, 160-180 rpm until OD600 is around 0.4; ② Transfer: Remove the phage Frozen vial, rapidly melt it in a 37 ℃ water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 37 ℃ and shake at 160-180rpm overnight for cultivation; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and filter it through a 0.22 μ m sterile filter. Collect the filtrate, which can be stored at 4 ℃ |
| Storage conditions | Liquid nitrogen |
| Sharing mode | Public welfare sharing |
Plasmid DNA of Infectious Hypodermal and Hematopoietic Necrosis Virus(Plasmid DNA of Infectious Hypodermal and Hematopoietic Necrosis Virus)
BNCC397428
Plasmid DNA of Peach Leaf Curl Virus (TSV)(Plasmid DNA of Peach Leaf Curl Virus (TSV))
BNCC397426
Plasmid DNA of Prawn yellow head virus(Plasmid DNA of Prawn yellow head virus)
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Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396427
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396428
Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398205
Lactobacillus intestinalis DNA standard
BNCC397806
Mycoplasma fermentans DNA lab quality control
BNCC397628
Plasmid DNA of Infectious myonecrosis virus (IMNV)
BNCC397427
Pichia kudriavzevii DNA lab quality control
BNCC397407
Human rhinovirus 61 RNA standard
BNCC397269
Genomic DNA from Bacillus cereus
BNCC397146
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