Escherichia coli bacteriophage vB|380692 |BNCC

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Escherichia phage vB_EcoP_AlfredRasser (Bas69) (Enquatrovirus N4)

Growth conditions	30 ℃; 18-24h; aerobic
Subculture procedure	phage culture: ① Host bacterial preparation: pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth culture medium, shake at 37 ℃, 160-180 rpm until OD600 is around 0.4; ② Transfer: Remove the phage Frozen vial, rapidly melt it in a 37 ℃ water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 37 ℃ and shake at 160-180rpm overnight for cultivation; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and filter it through a 0.22 μ m sterile filter. Collect the filtrate, which can be stored at 4 ℃
Storage conditions	Liquid nitrogen
Sharing mode	Public welfare sharing