Usutu virus|372733 |BNCC

BeNa Culture Collection

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  • Usutu virus-BNCC

Usutu virus

  • Price： $ 5572
  • number：BNCC372733
  • Packing：Frozen vial；3.1×10^5TCID50/mL
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Essential Information Related Products
Usutu virus
Culture medium BNCC361988
Description EMEM Complete Medium (with 2% FBS)
Composition 98% EMEM+2% FBS
Growth conditions 37°C;5% CO₂ + 95% air;
Subculture procedure ①Inoculation procedure: 24-48 hours before inoculation, transfer the frozen host cells into T25 flask, remove the cell culture medium when the host cell density reaches 80-90%, and inoculate 100-500 μL of virus solution. Adsorb under growth conditions for 1-2 hours, gently shake the T25 flask every 15-30 minutes to make the virus distribution more uniform; Add 6mL of virus growth medium to end adsorption, transfer to the incubator for further cultivation, and regularly observe the cytopathy; ② Virus collection: Once all host cells have developed lesions, virus liquid collection can begin. When collecting, the cells are repeatedly frozen and thawed 1-2 times, and then filtered through a 0.22 μ m pore size filter. The collected filtrate is the virus solution.
Storage conditions Liquid nitrogen
Sharing mode Public welfare sharing
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