Burgundy helices|370576 |BNCC

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Borrelia burgdorferi B31

Culture medium	BNCC390982
Description	ATCC Medium 1914
Composition	HEPES(Sigma H-3375):5.64g, Neopeptone:4.7g, Na₃C₆H₅O₇:0.7g, Glucose:5.64g, NaHCO₃:2.0g, TC-Yeast Extract(BD 255772):2.0g, C₃H₃NaO₃:0.75g, N-Acetylglucosamine:0.37g, Bovine Serum Albumin, Fraction V:47.0g, CMRL 1066 10X(without glutamine and sodium bicarbonate):100.0ml, Rabbit Serum(heat-inactivated):60.0ml, Deionized Water:840ml
Growth conditions	37 ℃; 7-10 days; 3% -5% O2, 10% CO2
Subculture procedure	① Dissolve the Frozen vial in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into a test tube containing 5-6mL of culture medium; ④ Then transfer to the above cultivation conditions for cultivation. ⑤ After 10 days of cultivation, the broth culture is not turbid, and there will be a slight precipitate at the bottom after centrifugation (Borrelia burgdorferi is a fragile and sensitive microorganism that requires the use of suitable culture medium for growth. Rabbit serum is crucial for the growth of this organism. The use of freshly prepared culture medium can promote growth. Culture media that have been prepared for more than a month should not be used.)
Storage conditions	-80 ℃
Sharing mode	Public welfare sharing