Toxoplasma gondii|385420 |BNCC

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Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux

Growth conditions	35-37 ℃; 5% CO2+95% air;
Subculture procedure	① Remove cryovials from liquid nitrogen or -80°C freezer, place in PE gloves, and quickly immerse in 37°C water bath. Shake cryovials to accelerate thawing; aim for complete thawing within 1 minute. ② Add the suspension to 6-7 mL of complete medium and incubate in a culture incubator. ③ Monitor medium pH and cell density. When cell density exceeds 2 × 10⁶ cells/mL, perform passage or cryopreservation.
Storage conditions	liquid nitrogen
Sharing mode	Public welfare sharing