Human herpesvirus 5|365213 |BNCC

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Human herpesvirus 5

Growth conditions	37 ℃; 5% CO2+95% air;
Subculture procedure	① Inoculation Steps: 24-48 hours before inoculation, lay T25 bottles of host cells and prepare for virus inoculation when the host cell density reaches 80-90%; Remove the cell culture medium and inoculate 100-500 μ L of virus solution, adsorb under culture conditions for 1-2 hours, gently shake the T25 bottle every 15-30 minutes to make the virus distribution more uniform; Add 6mL of virus growth medium to end adsorption, transfer to the incubator for further cultivation, and regularly observe the cell pathology; ② Virus collection: Once all host cells have developed lesions, virus liquid collection can begin. When collecting, the cells are repeatedly frozen and thawed 1-2 times, and then filtered through a 0.22 μ m pore size filter. The collected filtrate is the virus liquid
Storage conditions	Liquid Nitrogen
Sharing mode	Public welfare sharing