Human rhinovirus 30|381155 |BNCC

Essential Information Related Products

Human rhinovirus 30

Growth conditions	37 ℃; 7 days; 5% CO2+95% air;
Subculture procedure	① Inoculation steps: 24-48 hours before inoculation, lay T25 bottles of host cells and prepare for virus inoculation when the host cell density reaches 80-90%; Remove the cell culture medium and inoculate 100-500 μ L of virus solution, adsorb under culture conditions for 1-2 hours, gently shake the T25 bottle every 15-30 minutes to make the virus distribution more uniform; Add 6mL of virus growth medium to end adsorption, transfer to the incubator for further cultivation, and regularly observe the cell pathology; ② Virus collection: Once all host cells have developed lesions, virus liquid collection can begin. When collecting, the cells are repeatedly frozen and thawed 1-2 times, and then filtered through a 0.22 μ m pore size filter. The collected filtrate is the virus solution. The host cells are seeded in a 96 well plate and prepared for virus inoculation when the host cell density reaches 80-90%. ② Dilute the virus solution with complete culture medium in a 10 fold series, take 8 gradients of 10 ^ 1-10 ^ 8, and inoculate 100 μ L per well quantitatively. Inoculate 6 wells at each dilution and culture according to the culture conditions. Regularly observe and record the pathological changes of cells at each dilution
Storage conditions	liquid nitrogen
Sharing mode	Public welfare sharing