BeNa Culture Collection
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| Growth conditions | 37°C; 5% CO₂ + 95% Air |
| Subculture procedure | ①Inoculation procedure: 24-48 hours before inoculation, transfer the frozen host cells into T25 flask, remove the cell culture medium when the host cell density reaches 80-90%, and inoculate 100-500 μL of virus solution. Adsorb under growth conditions for 1-2 hours, gently shake the T25 flask every 15-30 minutes to make the virus distribution more uniform; Add 6mL of virus growth medium to end adsorption, transfer to the incubator for further cultivation, and regularly observe the cytopathy; ② Virus collection: Once all host cells have developed lesions, virus liquid collection can begin. When collecting, the cells are repeatedly frozen and thawed 1-2 times, and then filtered through a 0.22 μ m pore size filter. The collected filtrate is the virus solution. |
| Storage conditions | Liquid Nitrogen |
| Sharing mode | Public welfare sharing |
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396427
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396428
Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398205
Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398206
Plasmid DNA of White Spot Syndrome Virus (WSSV)(Plasmid DNA of White Spot Syndrome Virus (WSSV))
BNCC397425
Plasmid DNA of Necrotizing Hepatopancreatitis (NHP)(Plasmid DNA of Necrotizing Hepatopancreatitis (NHP))
BNCC397429
Sphingomonas sp. DNA lab quality control
BNCC398138
Mycoplasma fermentans DNA standard
BNCC397634
Mycoplasma bovis DNA lab quality control
BNCC397620
Human gammaherpesvirus 4 nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397551
Haemophilus influenzae nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397511
Escherichia coli DNA lab quality control
BNCC397356
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