Peripheral blood mononuclear cells|392533 |BNCC

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PBMC

Culture medium	BNCC379583
Description	P3HR-1 Cell-Specific Medium
Composition	80% RPMI-1640 + 20% Heat-Inactivated FBS
Growth conditions	37°C; 5% CO₂ + 95% air
Subculture procedure	Resuscitation steps: ① Remove the frozen vial from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves. Quickly immerse it in a 37 ℃ water bath, shake the vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete medium on a ultra-clean bench, centrifuge at 1000-1200rpm for 3-5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete medium. ③ Add the cell suspension into a T25 flask containing 6-7mL of complete medium and place the flask vertically in an incubator.
morphology	Single cells
Sharing mode	Public welfare sharing