CD4+(Untouched) (only available for cryopreservation)|384587 |BNCC

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CD4+(Untouched)

Culture medium	BNCC338360
Description	RPMI-1640 Complete Medium (with 10% FBS)
Composition	90%RPMI-1640+10%FBS
Growth conditions	37 ℃; 5% CO2+95% air;
Subculture procedure	Resuscitation steps: ① Remove the cryotube from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves, quickly immerse it in a 37 ℃ water bath, shake the cryotube to dissolve rapidly, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm for 3-5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Then add the cell suspension to a T25 bottle containing 6-7mL of complete culture medium and culture it in an incubator. It is recommended to cultivate the bottle vertically.
morphology	Lymphocyte like, single cell
Sharing mode	Public welfare sharing