Human acute myeloid leukemia cells|BNCC340568 |BNCC

Essential Information Related Products

MOLM-13

Culture medium	RPMI-1640 complete medium: 90% RPMI-1640 + 10% FBS
Subculture procedure	Recovery steps: ① Take out the frozen vial from liquid nitrogen or -80 ℃ refrigerator and put it into PE gloves, quickly submerse into a 37 ℃ water bath, shake the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min; (2) Add the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 3-5min, discard the supernatant, resuspend the cells with 1-2ml of complete media. ③The cell suspension was then added to the T25 flask containing 6-7mL of complete medium and placed in an incubator for culture. The culture flask is recommended to be cultured uprightly. cell subculture: ① centrifugation: collect cells, centrifuge at 1000rpm for 5min, discard the supernatant, add 1-2mL of culture solution and blow well, and dispense the cell suspension into a fresh T25 flask containing 8mL of culture medium according to a ratio of 1:2. (2) Half-volume medium exchange method: half-volume medium exchange method can be selected; Please gently suck half of the supernatant culture medium, resuspend and mix the remaining culture medium with cell precipitation, and divide the cell suspension into a fresh T25 flask containing 8mL culture medium according to a ratio of 1:2. (3) Pay attention to the change of pH of medium and cell density, renew the medium regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches more than 2 × 106 cells/ml.
Growth conditions	37 ℃;5% CO2 + 95% air;
Growth characteristics	Suspension growth
Storage conditions	Liquid nitrogen
Safety level	0
Sharing mode	Public welfare sharing